Double-digest genotype-by-sequencing of field and herbarium leaf tiss... - SRA

SRX9787963: Double-digest genotype-by-sequencing of field and herbarium leaf tissue of Paubrasilia echinata
1 ILLUMINA (Illumina HiSeq 4000) run: 5.6M spots, 525.6M bases, 182.5Mb downloads

Design: Library preparation and sequencing was carried out by the institute of integrative biology and systems (IBIS) at the University of Laval, Canada, using a modified GBS approach with single end reads and two restriction enzymes: a rare cutting enzyme PstI (CTGCAG) and a common cutting enzyme MspI (CCGG) (Poland et al., 2012).

Submitted by: Royal Botanical Gardens of Edinburgh

Study: Species delimitation and population dynamics of Paubrasilia echinata

PRJNA689870 • SRP300554 • All experiments • All runs

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1) Assess the population genetic structure of P. echinata by analysing a SNPs dataset generated by a double-digest GBS protocol (Elshire et al. 2011) from 38 samples of field collected and herbarium samples using various bioinformatics software;(2) Create and analyse a dataset of morphometric measurements from herbarium specimens from targeted loans, as well as digitized specimens;(3) Evaluate taxon limits by conducting multivariate analyses including genetic, morphological and ecological data to determine the extent to which different geno/morpho/ecotypes exist and should be formally recognised nomenclaturally.

Sample: herbarium_voucher

SAMN17221334 • SRS7974390 • All experiments • All runs

Organism: Paubrasilia echinata

Library:

Name: Paubrasilia_echinata_27

Instrument: Illumina HiSeq 4000

Strategy: RAD-Seq

Source: GENOMIC

Selection: Restriction Digest

Layout: SINGLE

Runs: 1 run, 5.6M spots, 525.6M bases, 182.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR13363863	5,591,072	525.6M	182.5Mb	2022-02-28

ID:: 12798777

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